FIGURE 2.

Gracillin induces apoptosis in human CRC cells. A, RKO and HCT116 cells were treated with gracillin at different concentrations (0, 2.5, 5.0 or 10.0 μmol/L) for 36 h. Cells were harvest and then stained with Annexin V‐FITC and PI, before being analysed by flow cytometry. Data are presented as means of three experiments, and error bars represent SD (*P < .05, **P < .01, ***P < .001). B, HCT116 and RKO cells were treated with different concentrations of gracillin (0, 2.5, 5.0 or 10.0 μmol/L) for 36 h. Cells were stained with Hoechst 33 258, and their apoptotic morphological characteristics were analysed. C, HCT116, RKO and SW480 cells were treated with gracillin at different concentrations (0, 2.5, 5.0 or 10.0 μmol/L) for 36 h. The expression levels of Bcl‐2, BAX, cleaved caspase‐3 and GAPDH were evaluated by Western blot analysis