FIGURE 5.

Gracillin inhibits CRC tumour growth in a xenograft model. A, BALB/c nude mice were administered gracillin intraperitoneally (1 or 2 mg/kg) every other day. Tumour volume (V) was calculated based on its length (A) and width (B), according to the following formula: V = 0.5 × A × B × B. (B) Tumour weight (n = 6). C, Representative images of the solid tumour in the control and gracillin‐treated groups. D, Mice total bodyweight. E, Proteins extracted from tumour tissues were evaluated by the Western blot assay. F, Tissue samples derived from the hearts, livers, kidneys and lungs of mice from the three groups and stained with H&E. All images were captured using light microscopy. Data are presented as means of three experiments, and error bars represent SD (*P < .05, ***P < .001, ****P < .0001)