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. 2020 Dec 7;25(2):1128–1139. doi: 10.1111/jcmm.16179

TABLE 2.

Antibodies used for Western blot analysis

Protein target Primary antibody Dilution used Antibody type Secondary antibody Dilution used Originated
P450scc Mouse anti‐P450scc 1/1000 Polyclonal anti‐mouse IgG 1/2000 Invitrogen, Carlsbad, CA, USA
P450c17 Mouse anti‐P450c17 1/1000 Polyclonal anti‐mouse IgG 1/2000 Invitrogen, Carlsbad, CA, USA
LH‐R Mouse anti‐LHR 1/1000 Monoclonal anti‐mouse IgG 1/2000 Invitrogen, Carlsbad, CA, USA

Abbreviations: P450c17, P45017α‐hydroxylase; P450scc, cholesterol side‐chain cleavage enzyme.