Figure 1.

OPG inhibits osteoclastogenesis by increasing the autophagy, and inactivation of PI3K/Akt and TAK1/S6 signalling pathways in primary osteoclasts and OCLs. A, The formation and number of TRAP‐positive multinucleated cells were counted treatment with different concentrations of OPG. B‐E, The expression of c‐Fos, MMP‐9, CAⅡ, Src, Beclin1, LC3II, p62, the PI3K/Akt and the TAK1/S6 signalling‐related proteins were detected incubation with different treatments. All values are the mean ± SD. ^** P < .01, ^* P < .05