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. 2020 Dec 6;25(2):960–974. doi: 10.1111/jcmm.16157

FIGURE 4.

FIGURE 4

Effect of C5aR blockade on gut microbiota homeostasis in db/db mice. (A and C) Representative images of H&E and PAS staining of colons from mice (scale bar = 80 µm). (B and D) Quantification of the villi length (µm) and goblet‐positive cells detected by H&E and PAS staining. (E and F) Measurement of Claudin 1 expression in the small intestine of mice by IHC staining (scale bar = 40 µm). (G) The Shannon index of gut microbiota in mice detected by 16S rRNA sequencing. (H) Principal co‐ordinates analysis (PCoA) of gut microbiota in mice using 16S rRNA sequencing. (I and K) Measurement of the gut microbiota abundance of mice at the phylum level. (J and L) Measurement of the gut microbiota abundance of mice at the genus level. (M) GC‐MS analysis of SCFAs (acetate, butyrate and propionate) level in faecal samples of mice.*P < .05, db/db or db/db + C5aRA group vs db/m group; # P < .05, db/db + C5aRA group vs db/db group