Figure 5.

NDUFA4L2 activation promoted proliferation of hypoxic HPASMCs. A, The efficiency and specificity of siRNA directed against NDUFA4L2 (si‐NDUFA4L2); (B) CCK‐8 was analysed the proliferation of HPASMCs under normoxia, hypoxia and hypoxia + si‐NDUFA4L2; (C) Cell cycles was analysed the proliferation of HPASMCs after knockdown of NDUFA4L2 in hypoxia condition; (D) The histogram showed the percentage of cells in the G₀/G₁, S and G₂/M phases; (E) EdU staining was analysed the proliferation of NDUFA4L2 in hypoxic HPASMCs; (F) Quantitative analysis of EdU‐positive staining; (G) Western blotting examined the expression of PCNA and cycle‐related protein after knockdown of NDUFA4L2 in hypoxia condition; (H) The histogram showed the expression results of cycle‐related protein. NC, negative control; Nor, normoxia; Hyp, hypoxia. Scale bar = 50 μm. ^** P < .01, ^*** P < .001. n = 6. All of the values are denoted as mean ± SEM