Table 5.
Digestion, Mutation, toxicity, allergenicity, hydro and physiochemical profiling of selected peptides
| Peptides | Non‐digesting enzymes | Mutation position | Toxicity | Allergenicity | Hydrophobicity | Hydrophilicity | Charge | PI | Mass | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| MHC‐I | 1 | KIADYNYKL | Clostripain,Cyanogen_Bromide,IodosoBenzoate,Proline_Endopept,Staph_Protease,Trypsin_R | NM | NT | A | −0.22 | 0.06 | 1 | 8.76 | 1127.31 |
| 2 | TNFTISVTT | Clostripain,Cyanogen_Bromide,IodosoBenzoate,Proline_Endopept,Staph_Protease,Trypsin_K,Trypsin_R,AspN | NM | NT | NA | 0.03 | −0.77 | 0 | 5.88 | 983.09 | |
| 3 | VVVLSFELL | Trypsin,Clostripain,Cyanogen_Bromide,IodosoBenzoate,Proline_Endopept,Trypsin_K,Trypsin_R,AspN | NM | NT | A | 0.33 | −1.01 | −1 | 4.00 | 1018.26 | |
| 4 | TLDSKTQSL | Chymotrypsin,Clostripain,Cyanogen_Bromide,IodosoBenzoate,Proline_Endopept,Staph_Protease,Trypsin_R | NM | NT | A | −0.26 | 0.27 | 0 | 6.19 | 992.09 | |
| 5 | GKQGNFKNL | Clostripain,Cyanogen_Bromide,IodosoBenzoate,Proline_Endopept,Staph_Protease,Trypsin_R,AspN ,Elastase | NM | NT | NA | −0.3 | 0.26 | 2 | 10.02 | 1005.14 | |
| 6 | VRDLPQGFS | Cyanogen_Bromide,IodosoBenzoate,Staph_Protease,Trypsin_K | NM | NT | NA | −0.18 | 0.08 | 0 | 6.19 | 1018.14 | |
| 7 | PWYIWLGFI | Trypsin,Clostripain,Cyanogen_Bromide,Staph_Protease,Trypsin_K,Trypsin_R,AspN | NM | NT | NA | 0.38 | −1.89 | 0 | 5.88 | 1194.44 | |
| 8 | NFGAISSVL | Trypsin,Clostripain,Cyanogen_Bromide,IodosoBenzoate,Proline_Endopept,Staph_Protease,Trypsin_K,Trypsin_R,AspN | NM | NT | NA | 0.18 | −0.81 | 0 | 5.88 | 907.03 | |
| 9 | QGFSALEPL | Trypsin,Clostripain,Cyanogen_Bromide,IodosoBenzoate,Trypsin_K,Trypsin_R,AspN | NM | NT | NA | 0.05 | −0.34 | −1 | 4.00 | 961.08 | |
| 10 | NHTSPDVDL | Trypsin,Chymotrypsin,Clostripain,Cyanogen_Bromide,IodosoBenzoate,Staph_Protease,Trypsin_K,Trypsin_R,Chymotrypsin(modified) | NM | NT | A | −0.21 | 0.26 | −1.5 | 4.20 | 997.03 | |
| MHC‐II | 1 | LEILDITPC | Trypsin,Chymotrypsin,Clostripain,Cyanogen_Bromide,IodosoBenzoate,Trypsin_K,Trypsin_R | NM | NT | A | 0.11 | −0.29 | −2 | 3.67 | 1016.22 |
| 2 | LPVSMTKTS | Chymotrypsin,IodosoBenzoate,Staph_Protease,Trypsin_R,AspN,Chymotrypsin(modified) | NM | NT | A | −0.08 | −0.2 | 1 | 9.11 | 963.16 | |
| 3 | VVFLHVTYV | Trypsin,Clostripain,Cyanogen_Bromide,IodosoBenzoate,Proline_Endopept,Staph_Protease,Trypsin_K,Trypsin_R,AspN | NM | NT | A | 0.3 | −1.5 | 0.5 | 7.09 | 1076.30 | |
| 4 | YYVGYLQPR | Trypsin,Clostripain,Cyanogen_Bromide,IodosoBenzoate,Staph_Protease,Trypsin_K,Trypsin_R,AspN | NM | NT | NA | −0.14 | −0.78 | 1 | 8.83 | 1158.32 | |
| 5 | VVLSFELLH | Trypsin,Clostripain,Cyanogen_Bromide,IodosoBenzoate,Proline_Endopept,Trypsin_K,Trypsin_R,AspN | NM | NT | NA | 0.22 | −0.9 | −0.5 | 5.25 | 1056.27 | |
| 6 | VVIGIVNNT | Trypsin,Chymotrypsin,Clostripain,Cyanogen_Bromide,IodosoBenzoate,Proline_Endopept,Staph_Protease,Trypsin_K,Trypsin_R,AspN,Chymotrypsin(modified) | NM | NT | NA | 0.2 | −0.9 | 0 | 5.88 | 928.10 | |
| 7 | YVGYLQPRT | Cyanogen_Bromide,IodosoBenzoate,Staph_Protease,Trypsin_K,AspN | NM | NT | NA | −0.16 | −0.57 | 1 | 8.93 | 1096.25 | |
| 8 | VNLTTRTQL | Chymotrypsin,Cyanogen_Bromide,IodosoBenzoate,Proline_Endopept,Staph_Protease,Trypsin_K,AspN | NM | NT | NA | −0.23 | −0.32 | 1 | 10.11 | 1045.20 | |
| 9 | IGINITRFQ | Cyanogen_Bromide,IodosoBenzoate,Proline_Endopept,Staph_Protease,Trypsin_K,AspN | NM | NT | NA | −0.03 | −0.54 | 1 | 10.11 | 1061.25 | |
| 10 | LVKNKCVNF | Chymotrypsin,Clostripain,Cyanogen_Bromide,IodosoBenzoate,Proline_Endopept,Staph_Protease,Trypsin_R,AspN | NM | NT | A | −0.14 | −0.21 | 2 | 9.36 | 1064.31 | |
Abbreviations: NA PROBABLE NO‐ALLERGEN, A PROBABLE ALLERGEN and the non‐digesting enzymes showing those enzymes which do not digest peptides into fragments; NM, No Mutation; NT, Non‐Toxin; T, toxic.