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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: J Exp Zool A Ecol Integr Physiol. 2020 Aug 15;333(8):550–560. doi: 10.1002/jez.2404

FIGURE 3.

FIGURE 3

(a) The region of the preoptic area (labeled here as MPO) used for the analysis is shown, with the region of interest indicated with a green outline. (b) A representative image of BrdU nuclear labeling in the POA of a DMSO female pup whose dam was injected with BrdU on E14. The region of interest outlined in green (a) is shown at ×20 magnification in (b). White scale bar = 25 μm. The brain map shown in (a) was modified from Paxinos (1991). BrdU, bromo-2-deoxy-5-uridine; DMSO, dimethyl sulfoxide; E14, Embryonic Day 14; MPO, medial preoptic area; POA, preoptic area