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. 2021 Jan 18;18(3):660–674. doi: 10.1038/s41423-020-00627-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Cell-surface expression of GPI-anchored antibodies in transduced TZM-bl cells. A Diagram of the lentiviral transfer vector expressing GPI-anchored antibodies. The encoding sequence of m36.4 or FluIgG03 scFv was genetically linked with sequences encoding a His tag and the GPI attachment signal of DAF. RRE, Rev response element; cPPT, central polypurine track; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element. B Expression of GPI-m36.4 or GPI-FluIgG03 on the surface of transduced TZM-bl cells with or without PI-PLC treatment, which was detected by an anti-His tag antibody and analyzed by FACS analysis. C Localization of GPI-m36.4 or GPI-FluIgG03 in transduced TZM-bl cells with confocal analysis. Alexa555-CtxB, cells stained with an Alexa Fluor 555-conjugated cholera toxin B subunit; Alexa488-Anti-His: cells stained with a mouse anti-His tag antibody followed by an Alexa Fluor 488-conjugated goat anti-mouse IgG antibody