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. 2021 Jan 7;21(3):186. doi: 10.3892/etm.2021.9617

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Protective effect of SFN on H₂O₂-treated HUVECs. (A-C) HUVECs were treated with 200 µmol H₂O₂, with or without 1.0 µmol/l SFN pretreatment. (A) Cell viability of HUVECs was detected using CellTiter-Blue^® assay. (B) Apoptosis was measured by flow cytometry. (C) Intracellular ROS levels were measured using the DCFH-DA probe. ^*P<0.05 vs. Control and ^#P<0.05 vs. H₂O₂. Data are presented as the mean ± standard deviation, n=5. SFN, sulforaphane; HUVECs, human umbilical vein endothelial cells; ROS, reactive oxygen species; DCFH-DA, 2',7'-dichlorofluorescin diacetate.