Figure 2.

SFN inhibits miR-34a expression but upregulates SIRT1 expression in HUVECs treated with H₂O₂. (A-D) HUVECs were treated with H₂O₂, with or without SFN pretreatment. (A) Changes in miR-34a (B) SIRT1 mRNA expression were measured using RT-qPCR. (C) SIRT1 protein expression was measured by western blot analysis before (D) the protein bands were quantified. ^*P<0.05 vs. Control; ^#P<0.05 vs. H₂O₂. Data are presented as the mean ± standard deviation, n=5. SFN, sulforaphane; miR, microRNA; SIRT1, sirtuin-1; HUVECs, human umbilical vein endothelial cells; RT-qPCR, reverse transcription-quantitative PCR.