Figure 3.

miR-34a suppresses SIRT1 mRNA expression by directly binding to its 3'-UTR. miR-34a mimics and inhibitors were transfected into human umbilical vein endothelial cells to change following which the effects of transfection on the level of (A) miR-34a and (B) SIRT1 mRNA expression were verified using reverse transcription-quantitative PCR. (C) Changes in the level of SIRT1 protein expression following transfection with the miR-34a mimic or miR-34a inhibitor were measured using western blot analysis and (D) quantified. (E) The binding capacity of miR-34a to the 3'-UTR of SIRT1 mRNA was verified by dual-luciferase reporter assay. ^*P<0.05 vs. Transfection-NC. Data are presented as the mean ± standard deviation, n=5. miR, microRNA; SIRT1, sirtuin-1; 3'-UTR, 3'-untranslated region; NC, negative control; WT, wild-type; MUT, mutant.