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. 2021 Jan 7;21(3):186. doi: 10.3892/etm.2021.9617

Figure 4.

Figure 4

Alteration of the miR-34a/SIRT1 axis influences HUVEC cell viability and apoptosis following H2O2 treatment. miR-34a mimics and inhibitors were transfected into HUVECs 24 h before H2O2 treatment. To change SIRT1 expression in cells, SIRT1-siRNA or pcDNA3.1-SIRT1 were transfected into HUVECs. HUVECs were treated with H2O2. (A) The effect of siRNA-SIRT1 and pcDNA3.1-SIRT1 transfection on SIRT1 mRNA expression was verified by reverse transcription-quantitative PCR analysis. (B) The effect of siRNA-SIRT1 and pcDNA3.1-SIRT1 transfection on SIRT1 protein expression was verified by western blot analysis and (C) quantified. (D and E) HUVEC cells were transfected with the indicated combination of miR-34a mimic, miR-34a inhibitor, pcDNA3.1-SIRT1 or siRNA-SIRT1, following which they were treated with H2O2. (D) HUVEC cell viability was measured after 48 h using CellTiter-Blue® assay. (E) HUVEC apoptosis was measured by flow cytometry. *P<0.05 vs. Transfection-NC; #P<0.05 vs. H2O2 + Transfection-NC. Data are presented as the mean ± standard deviation, n=5. miR, microRNA; SIRT1, sirtuin-1; HUVECs, human umbilical vein endothelial cells; siRNA, small interfering RNA; NC, negative control; Transfection-NC, co-transfection with the empty pcDNA3.1 vector and Allstar.