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. 2021 Jan 7;21(3):186. doi: 10.3892/etm.2021.9617

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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SFN inhibits miR-34a expression whilst enhancing SIRT1 expression by upregulating of Nrf2 in HUVECs. HUVECs were transfected with pcDNA3.1-Nrf2 or NC followed by treatment with SFN. (A) Changes in the level of Nrf2 expression, following treatment with SFN or transfection with pcDNA3.1-Nrf2 were measured using RT-qPCR analysis. (B) The effect of SFN or pcDNA3.1-Nrf2 transfection on Nrf2 protein expression was verified by western blot analysis and (C) the protein bands were quantified. (D) Changes in the expression levels of miR-34a and SIRT1 mRNA following Nrf2 overexpression were measured by RT-qPCR analysis. (E) The effect of pcDNA3.1-Nrf2 transfection on SIRT1 protein expression was verified using western blot analysis and (F) the protein bands were quantified. ^*P<0.05 vs. Transfection-NC. Data are presented as the mean ± standard deviation, n=5. SFN, sulforaphane; miR, microRNA; Nrf2, nuclear factor erythroid-2-related factor 2; HUVECs, human umbilical vein endothelial cells; RT-qPCR, reverse transcription-quantitative PCR; SIRT1, sirtuin-1; NC, negative control.