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Primer and protocols used for amplification of some vector-borne pathogens
| Organism | Target gene | Product size (bp) | PCR primers (5’-3’) | Reference |
|---|---|---|---|---|
| Babesia spp. | 18S rRNA | 422–440 |
F: GTTTCTGMCCCATCAGCTTGA R: CAAGACAAAAGTCTGCTTGAAAC |
[36] |
| Hepatozoon spp. | 18S rRNA | 666–800 |
F: ATACATGAGCAAAATCTCAAC R: CTTATTATTCCATGCAG |
[37] |
| Hemoplasmas | 16S rRNA | 595–618 |
F: ATACGGCCCATATTCCTACG R: TGCTCCACCACTTGTTCA |
[38] |
