CHEMICALS AND REAGENTS |
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BMS-34A3:C22 |
Sigma-Aldrich, Vienna, Austria |
Cat#: B9935 |
32P-γ-ATP |
Hartmann Analytic, Braunschweig, Germany |
Cat#: SRP-301H |
ANTI-FLAG M2 AFFINITY GEL |
Thermo Scientific, Vienna, Austria |
Cat#: A2220 |
Benzonase, Nuclease |
Sigma-Aldrich, Vienna, Austria |
Cat#: E1014-5KU |
CFSE Cell Division Tracker Kit |
BioLegend, CA, USA |
Cat#: 423801 |
cOmplete,Protease Inhibitor Cocktail |
Roche, Vienna, Austria |
Cat#: 11836170001 |
Dynabeads |
Thermo Scientific, Vienna, Austria |
Cat#: 1002D |
EdU HTS Kit 555 |
Sigma-Aldrich, Vienna, Austria |
Cat#: BCK-HTS555 |
FxCycle Violet Stain |
Thermo Scientific, Vienna, Austria |
Cat#: F10347
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Nano-Glo Luciferase Assay System |
Promega, Mannheim, Germany |
Cat#: N1110 |
NeutrAvidin-coated strips |
Pierce, Thermo Scientific, Vienna, Austria |
Cat#: 15127 |
PhastGel |
Thermo Scientific, Vienna, Austria |
Cat#: 10734927 |
PVDF-Membran |
Roth, Karlsruhe, Germany |
Cat#: T830.1 |
Pyrex(R) cloning cylinder |
Sigma-Aldrich, Vienna, Austria |
Cat#: CLS31666-125EA |
QuikChange mutagenesis kit |
Stratagene, Santa Clara, CA |
Cat#: 200518 |
Recombinant Human Proto-oncogene c-Myc |
RayBiotech, Georgia, USA |
Cat#: RB-230-00580-50 |
Skim Milk Powder |
Sigma-Aldrich, Vienna, Austria |
Cat#: 70166 |
SYTOX Green Nucleic Acid Stain |
Thermo Scientific, Vienna, Austria |
Cat#: S7020 |
TNFα |
Bio-Techne Ltd., Minnesota, USA |
Cat#: 210-TA-020 |
TurboFect |
Thermo Scientific, Vienna, Austria |
Cat#: R0531 |
WesternBright Chemilumineszenz Substrat |
Biozym, Vienna, Austria |
Cat#: 541021 |
ANTIBODIES |
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Anti-mouse IgG, HRP-linked Antibody (1:1000) |
Cell Signaling Technology, Frankfurt, Germany |
Cat#: 7076; RRID: AB_330924 |
Anti-rabbit IgG, HRP-linked Antibody (1:1000) |
Cell Signaling Technology, Frankfurt, Germany |
Cat#: 7074; RRID: AB_2099233 |
APC Annexin V (1:50) |
BioLegend, CA, USA |
Cat#: 640920 |
c-Myc/N-Myc (D3N8F) Rabbit mAb (1:1000) |
Cell Signaling Technology, Frankfurt, Germany |
Cat#: 13987; RRID: AB_2631168 |
Donkey Anti-Mouse IgG H&L (Dylight 550) (1:200) |
Abcam, Cambridge, UK |
Cat#: ab98795; RRID: AB_10675196 |
Donkey Anti-Rabbit IgG H&L (DyLight 650) (1:200) |
Abcam, Cambridge, UK |
Cat#: ab96922; RRID: AB_10680408 |
GAPDH (D16H11) XP® Rabbit mAb (1:1000) |
Cell Signaling Technology, Frankfurt, Germany |
Cat#: 5174; RRID: AB_10622025 |
GSK-3β (D5C5Z) XP® Rabbit mAb (1:1000) |
Cell Signaling Technology, Frankfurt, Germany |
Cat#: 12456; RRID: AB_2636978 |
IKKα (3G12) Mouse mAb (1:1000) |
Cell Signaling Technology, Frankfurt, Germany |
Cat#: 11930; RRID: AB_2687618 |
IKKα (B-8) mouse (1:50) |
Santa Cruz, CA, USA |
Cat#: sc-7606; RRID: AB_627784 |
IKKα (D3W6N) Rabbit mAb (1:200) |
Cell Signaling Technology, Frankfurt, Germany |
Cat#: 61294; RRID: AB_2799606 |
IKKβ (D30C6) Rabbit mAb (IF 1:100, WB 1:1000) |
Cell Signaling Technology, Frankfurt, Germany |
Cat#: RRID: AB_11024092 |
Phospho-c-Myc (Ser62) Polyclonal, IgG, Rabbit (1:200) |
Amsbio, Abington, UK |
AMS.E-AB-21265 |
Phospho-c-Myc (Thr58) Polyclonal, IgG, Rabbit (1:200) |
Amsbio, Abington, UK |
AMS.E-AB-20845 |
ENZYMES |
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BamHI |
New England Biolabs, Frankfurt, Germany |
Cat#: R3136 |
BbsI |
New England Biolabs, Frankfurt, Germany |
Cat#: R3539 |
HindIII |
New England Biolabs, Frankfurt, Germany |
Cat#: R3104 |
NheI |
New England Biolabs, Frankfurt, Germany |
Cat#: R3131 |
XbaI |
New England Biolabs, Frankfurt, Germany |
Cat#: R0145 |
Q5 High-Fidelity DNA Polymerase |
New England Biolabs, Frankfurt, Germany |
Cat#: M0491S |
PLASMIDS |
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pNL1.1 [Nluc] Vector |
Promega, Mannheim, Germany |
Cat#: N1001 |
pcDNA3 |
Invitrogen, A, USA |
Cat#: discontinued |
pEGFP-C1 |
Clonetech (now Takara Bio), CA, USA |
Cat#: discontinued |
pEYFP-C1 |
Clonetech (now Takara Bio), CA, USA |
Cat#: discontinued |
pmDsRed-C1 |
Clonetech (now Takara Bio), CA, USA |
Cat#: discontinued |
pSpCas9(BB)-2A-Puro (PX459) V2.0 |
Addgene, Massachusetts, USA |
Cat#: 62988; RRID: Addgene_62,988 |
PUB6/V5-His/LacZ |
Thermo Scientific, Vienna, Austria |
Cat#: V25020 |
SOFTWARE |
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CytExpert |
Beckman Coulter, Vienna, Austria |
RRID:SCR_017217 |
Genevestigator |
Nebion AG, Zurich, Switzerland |
RRID:SCR_002358 |
Graphpad Prism |
GraphPad Software, CA, USA |
RRID:SCR_002798 |
ImageJ/Fiji |
Open source |
RRID: SCR_003070 |