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Validation of the split-GFP–Prp8 intein-splicing assay. (A) The Prp8 intein-splicing assay based on split-GFP. GFP (200 nM) and GFP–Prp8i (200 nM) were used with DMF or cisplatin (40 µM) for fluorescence detection. n = 8. ***P < 0.001. (B) Dose–response fitting of inhibition of splicing of the GFP–Prp8i by cisplatin. GFP–Prp8i (200 nM) was used. Cisplatin was in twofold serial dilutions with concentrations ranging from 100 µM (30 µg/mL) to 0.78 µM (0.23 µg/mL). n = 3.
