Fig. 3.

EspA–EspA interaction network in the filament. (A) Each EspA subunit interacts with eight other subunit chains within the filament. The reference chain s strand 3 (blue) interacts with the neighboring chains r (light blue) and t (dark blue) from the same strand 3, with chains i (light orange), j (orange), and k (dark orange) from strand 4 and with chains C (light brown), D (brown), and E (dark brown) from strand 2. (B) Buried surface area of the EspA–EspA interfaces using the same chain reference and the color coding as in A. The other four interfaces made with the reference chain s (with chains k, t, D, and E) are symmetry-related to these four. (C) Mapping of the EspA–EspA interactions in the reference subunit (chain s strand 3, blue color). The interactions made by the eight neighboring subunits are mapped in the reference subunit using the same color code as in A.