Fig. 2.

The PCE failed to form blastemas. (A) In situ hybridization of and1/2, raldh2, msxb, runx2b, and lef1 in regenerative fins at 2 dpa showing that blastemas were not built in the PCE. (B) and1 and lef1 were not expressed in the PCE with two-hole excavations. (C) Histological analyses of ACE (n = 6/8) and PCE (n = 5/6). (D) H3P antibody staining (red) was used to examine cell proliferation in the ACE (n = 7/10) and PCE (n = 7/9) DAPI counterstaining (blue). (E) qRT-PCR results comparing the differences in the relative expression of regeneration-associated genes for the PCE over the ACE (Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001). Data are presented as mean ± SD. P, proximal; D, distal. (Scale bars: 200 µM in A and B; 100 µM in C and D.)