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. 2020 Dec 23;118(2):e2008861118. doi: 10.1073/pnas.2008861118

Fig. 5.

Fig. 5.

Perturbation of morphological maturation by dnMRTFA. (A) Design of dnMRTFA-GFP overexpression experiments. RPEL, actin-binding domain. (B) Basic region. QR, glutamine-rich region; SAP, SAP domain; LZ, leucine zipper domain. (B) In situ confocal optical sectioning of hearts with mosaic dnMRTFA-GFP expression. Hearts were perfused with Hoeschst dye and FM4-64 (scale bar, 20 µm). The arrows point to treated cells with defective T-tubule organization. (C) AutoTT quantification of T-tubule content of dnMRTFA-GFP expressing and nonexpressing cardiomyocytes. (D) Immunofluorescent staining of ACTN2 in isolated cardiomyocytes. (E) Quantification of Z-line distance. (F) Size and aspect ratio of dnMRTFA-GFP and control cardiomyocytes that were isolated from hearts with mosaic dnMRTFA expression. Box plots are described in Materials and Methods. Two-tailed Student’s t test versus control: *P < 0.05, **P < 0.01, ***P < 0.001.