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. 2021 Feb;27(2):202–220. doi: 10.1261/rna.078097.120

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© 2021 Seelam Prabhakar et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — (A) Probability distribution in the location of the side chain in the i⁶A hypermodified nucleobase at the 37th position in E. coli tRNA. (B) Overlays of i⁶A, io⁶A, or ms²i⁶A modified nucleobases in tRNA with respect to the nucleobase ring for structures taken at 5 ns intervals over the MD simulations. (C) Average structural deviation of anticodon nucleotides (RMSF, Å) over the MD simulations with respect to the initial structure for each i⁶A modified tRNA compared to unmodified A-tRNA.