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Microscopic visualization of inducible control of nuclear import with a TetR aptamer. (A) A3′SS-TN2-CP stably integrated into an HF1-3 cell line using the Flp-In system. (B,C) The generated cell line expressing the A3′SS-TN2-CP construct was transiently transfected with mCherry-tagged TetR (+) and treated with (+) or without (−) 50 µM doxycycline (dox) for 24 h. Cells were fixed and stained with DAPI. Scale bar, 10 µm.
