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CDK5RAP2-depeleted HeLa cells expressing EGFP-centrin1 and mCherry-NuMA with two centrosomes labeled with SiR-tubulin and observed with a 40× objective. Green and gray represent EGFP-centrin1 and SiR-tubulin, respectively. mCherry-NuMA is not shown. 10 × 2.2–μm z-stacks were acquired every 10 min. Playback is sped up 4,200× relative to real time. Image frames shown are maximum intensity projections at each time point. Related to Fig. S1 G.
