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. 2021 Jan 18;14:57. doi: 10.1186/s13071-020-04562-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — RNAi gene knockdown of Dg vATPase A in D. gallinae. Quantitative PCR gene expression analysis of Dg vATPase A expression in adult female D. gallinae mites fed on goose blood containing lacZ dsRNA (control) or Dg vATPase A dsRNA from R1 or R2. R1 and R2 Dg vATPase A dsRNAs were either added separately (a) or combined (b) in goose blood at a final concentration of 100 ng/μl, with the experiment shown in b containing equimolar amounts of R1 and R2 dsRNA. Dg vATPase A expression is shown at 96 h post-feed and is normalised to glyceraldehyde 3-phosphate dehydrogenase expression. Individual data points for biological replicates are shown with mean ± standard error of the mean (SEM) (n = 3–4). Asterisks represent significant difference at P < 0.05 between treatments determined by a one-way ANOVA with Dunnett’s multiple comparison test (a) or Student's t-test (b)