Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 3;3(1):vdaa165. doi: 10.1093/noajnl/vdaa165

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 1. — Activity of ST-401 and its analogues at the colchicine site of tubulin, and MT assembly and dynamics. (A) Diagram of MTA binding sites on tubulin. (B) ST-401 and its analogues are based on an N-ethyl-carbazole moiety (green) linked via a ketone (ST-34, ST-360, red) or a secondary alcohol (ST-377, ST-401, purple) to either a quinoline (ST-360, ST-401, blue) or a methyl-naphthalene residue (ST-34, ST-377, orange). (C) ST-compounds (5 μM) compete for [³H]colchicine-binding to tubulin. Results are mean ± SD from n = 3 experiments. Dotted line shows vehicle control (5% DMSO). (D) ST-compounds inhibit tubulin assembly measured by turbidity development of tubulin solutions. Representative IC₅₀ values from 3 experiments with comparable results. (E) ST-401 and ST-360 inhibit tubulin assembly measured in a pelleting assay. Results are mean ± SEM from 3 to 5 experiments. (F) ST-401 and nocodazole (NOC) reversibly inhibit MT assembly rates in HCT116 cells when comparing measures before (I), during (II), and after (III) treatment. N = 10–15 individual cells.