Figure 1.

Male and female USVs to familiar or unfamiliar females. (a) Schematic diagram of the experimental procedure. (b) Female USVs to the familiar or unfamiliar females under the condition of 30 min isolation before recording 1. (c) Female USVs to the familiar or unfamiliar females under the condition of 1 day isolation before recording 1. (d) Male USVs to the familiar or unfamiliar females under the condition of 30 min isolation before recording 1. (e) Male USVs to familiar or unfamiliar females under the condition of 1 day isolation before recording 1. *p < 0.05, Wilcoxon signed-rank test; (b), n = 11; (c), n = 14, (d and e), n = 15. Counter-balanced order of experimental contents for recording 1 and 2 in each individual is indicated as follows: blue open circle, recording 1 with familiar and recording 2 with unfamiliar female (fam–unfam); lime yellow closed square, recording 1 with unfamiliar and recording 2 with familiar female (unfam–fam).