Table 2.
Physico-chemical characteristics of scaffolds and in vitro MTT assay of L929 fibroblasts after 7 days.
| nanoscaffolds | pore diameter (µm) | fibre diameter (µm) | water contact angle (°) | Young's modulus (MPa) | number of cells as determined from MTT assay |
|---|---|---|---|---|---|
| polyhydroxybutyrate (PHB)/kappa-carrageenan (KCG) | |||||
| 100/0 | 1.9 ± 0.7 | 1.3 ± 0.4 | 126 ± 1 | 518.2 ± 36.5 | 56 068 ± 3498 |
| 90/10 | 1.2 ± 0.6 | 0.9 ± 0.5 | 120 ± 1.9 | 271.7 ± 58.9 | 49 515 ± 10 347 |
| 80/20 | 0.9 ± 0.3 | 0.7 ± 0.5 | 107 ± 1.3 | 160.5 ± 32.8 | 35 661 ± 3079 |
| 70/30 | 1.1 ± 0.5 | 0.7 ± 0.5 | 104 ± 0.9 | 90.6 ± 10.9 | 38 083 ± 11 082 |
| poly(hydroxybutyrate-co-valerate) (PHBV)/KCG | |||||
| 100/0 | 0.8 ± 0.3 | 0.4 ± 0.1 | 112 ± 1 | 197.2 ± 44.6 | 73 421 ± 3834 |
| 90/10 | 1.0 ± 0.5 | 0.7 ± 0.2 | 73.6 ± 2.8 | 160 ± 14.8 | 59 800 ± 17 584 |
| 80/20 | 0.9 ± 0.3 | 0.6 ± 0.2 | 62.4 ± 0.5 | 111.7 ± 22.2 | 45 060 ± 12 110 |
| 70/30 | 1.0 ± 0.5 | 0.5 ± 0.2 | 57.8 ± 1.7 | 108.5 ± 6.8 | 46 109 ± 10 857 |
| polydioxanone (PDX)/fucoidan (FUC) | |||||
| 100/0 | 0.2 ± 0.06 | 0.3 ± 0.1 | 32.1 ± 0.0 | 73.8 ± 7.6 | 32 372 ± 6793 |
| 90/10 | 0.2 ± 0.08 | 0.2 ± 0.07 | 32.1 ± 0.0 | 69.6 ± 8.4 | 7510 ± 4328 |
| 80/20 | 0.2 ± 0.1 | 0.2 ± 0.05 | 32.1 ± 0.0 | 38.1 ± 3.9 | 13 691 ± 4154 |
| 70/30 | 0.2 ± 0.1 | 0.2 ± 0.05 | 32.1 ± 0.0 | 35 ± 11.3 | 21 154 ± 6873 |
| PDX/KCG | |||||
| 100/0 | 2.0 ± 0.7 | 1.1 ± 0.3 | 32.1 ± 0.0 | 73.8 ± 7.6 | 32 372 ± 6793 |
| 90/10 | 1.6 ± 0.5 | 1.0 ± 0.2 | 32.1 ± 0.0 | 72 ± 6.5 | 21 831 ± 6967 |
| 80/20 | 1.5 ± 0.6 | 0.9 ± 0.2 | 32.1 ± 0.0 | 42.6 ± 6.3 | 16 606 ± 3756 |
| 70/30 | 0.9 ± 0.3 | 0.5 ± 0.2 | 32.1 ± 0.0 | 38.2 ± 5.5 | 30 576 ± 3825 |
| PDX/PHBV | |||||
| 100/0 | 1.1 ± 0.4 | 0.4 ± 0.1 | 32.1 ± 0.0 | 73.8 ± 7.6 | 24 956 ± 1969 |
| 90/10 | 1.7 ± 0.6 | 1.0 ± 0.3 | 32.1 ± 0.0 | 95.6 ± 11.6 | 25 072 ± 4328 |
| 80/20 | 1.2 ± 0.4 | 0.7 ± 0.3 | 105.1 ± 2.2 | 72.9 ± 6.9 | 23 136 ± 6132 |
| 70/30 | 1.2 ± 0.5 | 0.6 ± 0.2 | 119.6 ± 2.5 | 100.4 ± 18.3 | 25 912 ± 9796 |
| PDX/PSuc | |||||
| 100/0 | 8.9 ± 5.4 | 1.0 ± 0.04 | 32.1 ± 0.0 | 51.8 ± 10.3 | 35 708 ± 6551 |
| 90/10 | 6.2 ± 4.2 | 0.8 ± 0.1 | 32.1 ± 0.0 | 43.0 ± 10.4 | 33 721 ± 17 362 |
| 80/20 | 5.1 ± 2.8 | 0.8 ± 0.03 | 32.1 ± 0.0 | 31.5 ± 5.2 | 57 370 ± 31 770 |
| 70/30 | 4.1 ± 3.2 | 0.7 ± 0.06 | 32.1 ± 0.0 | 83.2 ± 23.9 | 41 445 ± 14 515 |
| 60/40 | 3.6 ± 2.3 | 0.7 ± 0.03 | 32.1 ± 0.0 | 58.2 ± 27.5 | 10 1115 ± 73 374 |
| 50/50 | 3.4 ± 2.5 | 0.6 ± 0.04 | 32.1 ± 0.0 | 33.0 ± 2.0 | 18 131 ± 13 203 |
| PLLA/PSuc | |||||
| 100/0 | 5.7 ± 3.3 | 1.0 ± 0.05 | 141.3 ± 2.0 | 235 ± 15 | 51 408 ± 17 250 |
| 90/10 | 4.2 ± 2.5 | 0.9 ± 0.1 | 135.1 ± 1.6 | 109 ± 8.7 | 53 312 ± 13 995 |
| 80/20 | 3.9 ± 2.4 | 0.8 ± 0.03 | 134.0 ± 0.7 | 114.3 ± 4.0 | 56 111 ± 14 113 |
| 70/30 | 2.9 ± 2.0 | 0.7 ± 0.04 | 126.3 ± 4.7 | 85.7 ± 5.9 | 51 717 ± 31 051 |
| 60/40 | 2.01 ± 0.8 | 0.7 ± 0.01 | 132.9 ± 1.1 | 90.7 ± 5.5 | 49 338 ± 10 401 |
| 50/50 | 2.4 ± 1.5 | 0.6 ± 0.02 | 81.6 ± 9.5 | 99.3 ± 31.6 | 41 473 ± 12 501 |
| PLLA/CA | |||||
| 0/100 | 0.83 ± 0.3 | 0.7 ± 0.08 | 134.4 ± 1.8 | 90.4 ± 15.3 | 10 043 ± 6267 |
| 100/0 | 5.7 ± 3.3 | 1.0 ± 0.3 | 140.7 ± 1.8 | 235.4 ± 14.8 | 51 408 ± 17 450 |
| 90/10 | 2.3 ± 0.4 | 0.9 ± 0.3 | 138.4 ± 0.8 | 251.8 ± 23.4 | 53 228 ± 25 665 |
| 80/20 | 2.7 ± 0.5 | 0.8 ± 0.4 | 139.6 ± 0.7 | 173.3 ± 29.2 | 18 719 ± 4141 |
| 70/30 | 3.5 ± 1.1 | 0.6 ± 0.2 | 119.5 ± 0.9 | 58.9 ± 3.3 | 17 040 ± 7452 |
| 60/40 | 2.5 ± 0.7 | 0.5 ± 0.3 | 127.7 ± 0.7 | 41.2 ± 17.6 | 22 442 ± 5173 |
| 50/50 | 2.0 ± 0.6 | 0.5 ± 0.2 | 117.3 ± 1.3 | 104.4 ± 13.8 | 7552 ± 3618 |
| PLLA/cellulose | |||||
| 0/100 | 1.5 ± 0.6 | 0.3 ± 0.1 | 25.0 ± 0.0 | 162.3 ± 21.3 | 31 678 ± 14 856 |
| 100/0 | 5.7 ± 3.3 | 1.0 ± 0.3 | 140.7 ± 1.8 | 235.4 ± 14.8 | 51 408 ± 17 450 |
| 90/10 | 2.3 ± 0.4 | 0.8 ± 0.3 | 97.2 ± 0.4 | 275.0 ± 36.8 | 34 028 ± 17 333 |
| 80/20 | 2.7 ± 0.3 | 0.7 ± 0.2 | 25.0 ± 0.0 | 146.5 ± 10.6 | 34 420 ± 20 453 |
| 70/30 | 3.5 ± 1.0 | 0.6 ± 0.2 | 25.0 ± 0.0 | 95.7 ± 6.7 | 33 161 ± 23 589 |
| 60/40 | 3.0 ± 0.5 | 0.4 ± 0.2 | 25.0 ± 0.0 | 27.6 ± 12.4 | 30 444 ± 21 740 |
| 50/50 | 2.3 ± 0.5 | 0.3 ± 0.07 | 25.0 ± 0.0 | 102.6 ± 72.8 | 32 237 ± 11 258 |
| PDX/CA | |||||
| 100/0 | 8.9 ± 5.4 | 1.2 ± 0.5 | 32.1 ± 0.0 | 51.8 ± 10.3 | 35 708 ± 6551 |
| 90/10 | 3.2 ± 1.5 | 1.0 ± 0.3 | 32.1 ± 0.0 | 45.8 ± 4.6 | 45 280 ± 3130 |
| 80/20 | 2.6 ± 1.0 | 1.0 ± 0.5 | 32.1 ± 0.0 | 90.9 ± 16.0 | 35 904 ± 3914 |
| 70/30 | 4.8 ± 2.1 | 0.8 ± 0.3 | 32.1 ± 0.0 | 134.5 ± 15.5 | 33 665 ± 24 790 |
| 60/40 | 1.7 ± 0.6 | 0.6 ± 0.3 | 32.1 ± 0.0 | 191.8 ± 24.0 | 16 620 ± 8480 |
| 50/50 | 2.2 ± 0.5 | 0.6 ± 0.2 | 32.1 ± 0.0 | 65.7 ± 14.0 | 30 446 ± 10 504 |
| PLLA/CA 1% nanosilica | |||||
| 100/0 | 5.7 ± 3.3 | 1.0 ± 0.1 | 128.6 ± 0.8 | 168.3 ± 16.4 | 58 350 ± 9922 |
| 90/10 | 2.3 ± 0.4 | 0.6 ± 0.1 | 129.8 ± 1.3 | 129.4 ± 8.1 | 30 530 ± 12 091 |
| 70/30 | 2.9 ± 0.9 | 0.4 ± 0.1 | 130.4 ± 1.3 | 66.9 ± 8.9 | 15 333 ± 6890 |
| 50/50 | 2.4 ± 0.5 | 0.4 ± 0.1 | 123.6 ± 0.4 | 89.2 ± 10.3 | 9623 ± 9606 |
| PLLA/cellulose 1% nanosilica | |||||
| 100/0 | 5.7 ± 3.3 | 1.0 ± 0.1 | 128.6 ± 0.8 | 168.3 ± 16.4 | 58 350 ± 9922 |
| 90/10 | 2.3 ± 0.5 | 0.7 ± 0.1 | 108.7 ± 7.7 | 76.0 ± 14.0 | 41 473 ± 9204 |
| 70/30 | 2.2 ± 0.4 | 0.4 ± 0.1 | 25.0 ± 0.0 | 48.4 ± 20.8 | 41 165 ± 18 776 |
| 50/50 | 1.9 ± 0.5 | 0.3 ± 0.1 | 25.0 ± 0.0 | 135.1 ± 41.7 | 30 726 ± 11 732 |
| PDX/CA 1% nanosilica | |||||
| 100/0 | 1.4 ± 0.4 | 0.8 ± 0.1 | 32.1 ± 0.0 | 60.4 ± 8.4 | 50 485 ± 12 176 |
| 90/10 | 2.5 ± 0.9 | 1.0 ± 0.1 | 32.1 ± 0.0 | 68.1 ± 7.9 | 67 026 ± 15 791 |
| 60/40 | 2.5 ± 0.9 | 0.6 ± 0.1 | 32.1 ± 0.0 | 155.2 ± 5.0 | 35 036 ± 21 101 |