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. 2021 Jan 17;9(1):e001895. doi: 10.1136/jitc-2020-001895

Figure 1.

Figure 1

PD-L1 mRNA level is associated with CD8+ T cells infiltration and prognosis in MSS CRC. (A) Representative expression of PD-L1 protein and mRNA in MSS and MSI-H CRC tissues (scale bar, 100 µm; magnification scale bar, 200 µm). (B) Statistical analysis was conducted based on the level of PD-L1 protein and mRNA in patients with MSS and MSI-H. (C) Kaplan-Meier analysis of the overall survival rate of patients with MSS CRC, according to PD-L1 protein expression (left panel) and PD-L1 mRNA level (right panel). (D) Correlations of CD8+ cells densities with PD-L1 expression, according to PD-L1 protein expression (left panel) and PD-L1 mRNA level (right panel) in patients with MSS CRC (n=123). (E) Western Blotting detection showed that PD-L1 protein expression in MSS CRC tissues was higher than that in adjacent tissues (n=22). (F) RT-qPCR analysis showed that there was no significant difference in PD-L1 mRNA levels between MSS CRC tissues and adjacent tissues (n=54). P values and R values were calculated based on the analysis of Pearson’s correlation. The significance of survival difference was determined by the log rank test. Student’s t test. *P<0.05, **p<0.01, and ***p<0.001. CRC, colorectal cancer; MSI-H, microsatellite instability-high; MSS, microsatellite stable; PD-L1, programmed cell death ligand 1.