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. 2021 Jan 17;9(1):e001895. doi: 10.1136/jitc-2020-001895

Figure 5.

Figure 5

NRF1 is the major transcription factor for IL-17A to accumulate PD-L1 protein. (A) Schematic representation of predicted NRF1 and YY1 binding sites in the promoter of mouse miR-15b-5p. (B) Ch-IP analyzes of NRF1 or YY1 binding to the miR-15b-5p promoter by antibodies against NRF1 or YY1 in CT26 and MC38 cells. (C, D) Effects of NRF1/YY1 overexpression or knockdown on miR-15b-5p expression through RT-qPCR analysis. (E, F) Modulation of the miR-15b-5p promoter activity by NRF1 overexpression or knockdown in CT26 cells. (G) The regulation of IL-17A/P65 signaling on NRF1 and PD-L1 expression in CT26 and MC38 cells. (H) The effect of the IL-17A/P65/NRF1 axis on miR-15b-5p expression in CT26 cells. Data represent mean±SD. Results are representative of at least three separate experiments. Student’s t test. *P<0.05, **p<0.01, and ***p<0.001. Ch-IP, chromatin immunoprecipitation; NRF1, nuclear respiratory factor 1; PD-L1, programmed cell death ligand 1.