Figure 3.

Ectopic miR-760 expression inhibits the growth and invasion of GBM cells in vitro. (A) miR-760 expression in GBM cell lines (A172, U251, U138 and T98) and a normal human astrocyte cell line (NHA) was detected by RT-qPCR analysis. (B) U251 and T98 cells transfected with mIR-760 mimic or miR-NC were harvested and analyzed with RT-qPCR to evaluate the transfection efficiency of miR-760 mimic. (C, D) Cell Counting Kit-8 assay and flow cytometric analysis were conducted to determine the proliferation and apoptosis of miR-760-overexpressing U251 and T98 cells, respectively. (E, F) The migration and invasion of U251 and T98 cells with miR-760 mimic or miR-NC transfection were examined by Transwell assays. **P < 0.01.

Abbreviations: GBM, glioblastoma; RT-qPCR, reverse transcription-quantitative PCR; miR-NC, negative control miRNA mimic; miR, microRNA.