Figure 5.

Inhibition of miR-760 eliminates the effects of si-UBA6-AS1 on the proliferation, apoptosis, migration and invasion of GBM cells. (A) Reverse transcription-quantitative PCR analysis was used to measure the expression of miR-760 in U251 and T98 cells following miR-760 inhibitor or NC inhibitor transfection. (B, C) miR-760 inhibitor or NC inhibitor along with si-UBA6-AS1 were transfected into U251 and T98 cells. The proliferation and apoptosis of co-transfected cells were assessed by Cell Counting Kit-8 assay and flow cytometric analysis, respectively. (D, E) Transwell migration and invasion assays were used to evaluate the migration and invasion abilities of U251 and T98 cells treated as described above. *P < 0.05 and **P < 0.01.

Abbreviations: GBM, glioblastoma; NC inhibitor, negative control inhibitor; UBA6-AS1, UBA6 antisense RNA 1; si-NC, negative control small interfering RNA; si-UBA6-AS1, small interfering RNA targeting UBA6-AS1.