Figure 7.

UBA6-AS1 depletion inhibits GBM growth in vivo. (A) Representative images of tumor xenografts harvested from the sh-UBA6-AS1 and sh-NC groups. (B) Tumor growth curves were generated according to the tumor volumes monitored weekly for 4 weeks. (C) The weight of the tumor xenografts dissected from the sh-UBA6-AS1 and sh-NC groups was determined. (D) RT-qPCR analysis was used to measure UBA6-AS1 expression in the tumor xenografts from the sh-UBA6-AS1 and sh-NC groups. (E) HOXA2 protein expression in tumor xenografts from the sh-UBA6-AS1 and sh-NC groups was measured by Western blotting. (F) RT-qPCR analysis was used to measure miR-760 expression in tumor xenografts from the sh-UBA6-AS1 and sh-NC groups. **P < 0.01.

Abbreviations: GBM, glioblastoma; RT-qPCR, reverse transcription-quantitative PCR; HOXA2, homeobox A2; UBA6-AS1, UBA6 antisense RNA 1; sh-NC, negative control short-hairpin RNA; sh-UBA6-AS1, short-hairpin RNA targeting UBA6-AS1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; miR, microRNA.