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. 2021 Jan 14;13:367–377. doi: 10.2147/CMAR.S277399

Figure 2.

Figure 2

UCA1 knockdown enhances the toxicity of cisplatin to gastric cancer cells. (A) CCK8 kit was used to detect the cell viability after stimulating with 15 μg/mL cisplatin; (B–C) Small interfering RNA-UCA1 (si-UCA1) successfully knocked down UCA1 expression in gastric cancer cells verified by qPCR (B) and cellular immunofluorescence (C); (D–E) UCA1 knockdown by si-UCA1 increased the toxicity of cisplatin to AGS (D) and NCI-N87 (E, F) immunoblotting was used to detected the expression of caspase-3 and cleaved caspase-3 protein in human gastric cancer cell. Each experiment was repeated three times independently, ns was p>0.05, **p<0.01, ***p<0.001 vs si-NC group. The cell viability and UCA1 expression were shown as mean ±SD, and p-value was calculated by post hoc comparisons in (B) and by Student’s t-est in (D and E). Scale bar=50 μm.