. 2021 Jan 14;13:367–377. doi: 10.2147/CMAR.S277399

Table 1.

Sequence of Primers for qPCR, siRNA and miRNA Regulation

Primers Used in qPCR Analysis (5ʹ-3ʹ)
UCA1	Forward: CTCTCCATTGGGTTCACCATTC
UCA1	Reverse: GCGGCAGGTCTTAAGAGATGAG
miR-513a-3p	Forward: TAAATTTCACCTTTCTGAGAAGG
miR-513a-3p	Reverse: GCGAGCACAGAATTAATACGAC
CYP1B1	Forward: ACGTACCGGCCACTATCACT
CYP1B1	Reverse: CTCCCCACGACCTGATCCA
U6	Forward: AUAAAUCCCUUUACACCUCTT
U6	Reverse: AAUAAAUCCCUUUACACCUCTT
β-actin	Forward: AGCCCATCCTTCGAGTACAAA
β-actin	Reverse: TCTTGGTGCGATAACTGGTGG
si-RNA sequence (5ʹ-3ʹ)
si-NC	CAGAUGGCCACCAGUAACUGU
si-UCA1-1	AGUUAAAGGUGGUUAUCUGUU
si-UCA1-2	ACAGUUAAAGGUGGUUAUCUG
si-UCA1-3	UAUAGAAGACCACCUAAACAG
si-CYP1B1
miRNA regulate sequence (5ʹ-3ʹ)
miR-513a-3p-NC	AUUCGGGUUAAGCUAGAAGGUUC
miR-513a-3p-mimic	UAAAUUUCACCUUUCUGAGAAGG
miR-513a-3p-inhibitor	UCGAAGUGUCCCUCCACAGUAGU