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. 2021 Jan 14;16:371–381. doi: 10.2147/IJN.S287875

Figure 4.

Figure 4

Dia-Exos exhibit high levels of miR-20b-5p expression. (A) miR-20b-5p expression in diabetes patients and controls (n=10/group); (B) miR-20b-5p levels in patients with non-diabetic foot wounds and DFUs (n=10/group); (C) agomiR-20b-5p treatment enhanced miR-20b-5p expression by HSFs, as shown via qPCR; (D) the impact of agomiR-20b-5p on HSF proliferation was quantified via CCK-8 assay; (E) treatment with agomiR-20b-5p suppressed Cyclin D1 and Cyclin D3 expression; (F) qPCR was used to examine the impact of agomiR-20b-5p on t Bcl-2 and Bax expression; (G) collagen-related markers were evaluated to gauge the impact of miR-20b-5p on collagen synthesis; (H and I) ELISAs were used to measure bFGF and VEGFA secretion by HSFs treated as indicated. Data are means ± SD from triplicate experiments. *p<0.05, **p<0.01, ***p<0.001.