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. 2021 Jan 14;16:371–381. doi: 10.2147/IJN.S287875

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© 2021 Chen et al.

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MiR-20b-5p suppresses VEGFA expression and thereby controls HSF functionality. (A) miRDB, TargetScan, and miRWalk were utilized to identify possible targets of miR-20b-5p; (B) a luciferase reporter assay was used to examine the interactions between miR-20b-5p and VEGFA, with data being analyzed via the SpectraMax M5 software; (C and D) the impact of miR-20b-5p on VEGFA expression was quantified via qPCR and Western blotting; (E) a CCK-8 assay revealed that miR-20b-5p inhibition was sufficient to partially suppress the anti-proliferative impact of siRNA-mediated VEGFA knockdown in HSFs; (F) qPCR was used to measure the expression of Cyclin D1 and Cyclin D3; (G) the impact of VEFA expression on collagen synthesis in HSFs was evaluated based upon collagen-related marker expression; (H and I) ELISAs were used to measure bFGF and VEGFA secretion by HSFs treated as indicated. Data are means ± SD from triplicate experiments. *p<0.05, **p<0.01, ***p<0.001.