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. 2021 Jan 18;295:198307. doi: 10.1016/j.virusres.2021.198307

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Fig. 1 — Generation of cells stably expressing civet, bat and human ACE2. (A) Detection of ACE2 expression in HeLa cells by western-blot using anti-HA tag antibodies. Lanes 1 to 3: transiently transfectedcells expressing civet (c), bat (b) and human (h) ACE2; Lane 4: HeLa cell acts as a negative control; Lanes 5 to 7: cell lines stably expressing civet, bat and human ACE2 cell. (B) HeLa cells stably expressing civet, bat and human ACE2 were verified by immunofluorescence. ACE2 expression was detected with mouse anti-HA antibody followed by Alexa 555-conjugated goat anti-mouse IgG. Nuclei were stained with DAPI (6-diamidino-2-phenylindole). The top row shows HeLa cells as a negative control. Rows 2 to 4 show HeLa cells stably expressing hACE2, bACE2, and cACE2. The columns (from left to right) show cells of brightfield, staining of cell nuclei (blue), staining of expressed ACE2-HA (red fluorescence of Alexa 555), and the merged double-stained image.