Fig. 6.

The effects of AG490, phenethyl isothiocyanate (PEITC), and black radish extract (BRE) on lipopolysaccharides (LPS)-induced signal transducer and activator of transcription 3 (STAT3) phosphorylation. The RAW 264.7 cells were pre-treated with different concentrations of AG490, PEITC, and BRE for 30 min and then treated with LPS (1 μg/mL) for 4 h. Representative pictures of Western blot and quantitative analyses. Each immunoreactive band was digitized and expressed as a ratio of STAT3 protein levels. Data are expressed as mean±SD of the three independent experiments. ^##P<0.01 versus control; ^*P<0.05 and ^**P<0.01 versus LPS treatment alone.