Figure 6.

Activation of NET production by RA SF. (A) IPA predicted activation of signaling cascades regulated by AKT, RAF1 and SRC which may regulate NET production. (B) Neutrophils were incubated for 4 h with PMA (50 nM), A23187 (3.8 uM), or 10% RA SF. NET production was increased by RA SF as measured by quantification of extracellular DNA in culture supernatants, DAPI staining of extracellular NET DNA (blue), and immunofluorescent staining for myeloperoxidase (MPO, red) and citrullinated histone H3 (cit-H3, green) on NET structures (*p < 0.05, **p < 0.01, ***p < 0.001). White arrows indicate examples of NETs on each panel.