Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 5;8:594509. doi: 10.3389/fcell.2020.594509

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Jing, Lin, Du, Jiang, Li, Wang, Liu, Cui and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Cartilage changes and immunohistochemistry for matrix degrading enzymes ADAMTS5 and matrix metallopeptidase 13 (MMP-13) in Ctrl group and iron overloaded (IO) group. (A) Haematoxylin-Fast Green-Safranin-O staining of the medial femoral condyle and tibial plateau of control (n = 5) and iron overloaded (n = 5) mice at 2 months after surgical induction of OA. Iron overloaded mice showed a higher level of cartilage degeneration and higher OARSI scores in the right knees compared to the control group, while sham operated left knees of both two groups showed no significant cartilage destruction. (B) The Osteoarthritis Research Society International (OARSI) scores for the Sham and DMM operated knees of Ctrl group and iron overloaded (IO) group. Scale bar=200 μM; Data are presented as mean ± SD. *P < 0.05; **P < 0.01; ****P < 0.0001. EZ=0.838 for (B). Immunohistochemistry for matrix degrading enzymes ADAMTS5 (C) and matrix metallopeptidase 13 (MMP-13) (E) in the medial tibial plateau at 2 months after surgical induction of OA in Ctrl group (n = 4) and iron overloaded group (n = 4). The ratio of positive cells for ADAMTS5 (D) and MMP13 (F) were quantified under a microscope at 40X magnification using five sections from four mice. Scale bar=50 μM; Data are presented as mean ± SD. *P < 0.05; **P < 0.01. EZ=0.826 for (D); EZ=0.836 for (F).