Figure 7.

Inhibition of DMT1 suppressed IL-1β induced MAPK and PI3K/AKT/NFκB pathway activation. Chondrocytes were transfected with DMT1 siRNA, then treated with IL-1β for 30 min. The protein levels of P-P38, P38, ERK, P-ERK, JNK, P-JNK,P-AKT, AKT, P-PI3K, PI3K were determined by western blot (A,C) and quantification analysis (B,D). (E,F) Chondrocytes were transfected with DMT1 siRNA, then treated with IL-1β for 15 min. The protein levels of P-P65, P65 were determined by western blot and the band density were quantified and normalized to control. Data are presented as mean ± SD. All experiments were repeated three times independently. *P < 0.05; ***P < 0.001 vs. NC; ^#P < 0.05; ^##P < 0.01; ^###P < 0.001 vs. IL-1β treatment group.