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. 2020 Dec 5;413(4):1203–1214. doi: 10.1007/s00216-020-03084-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Comparison of nephrin imaging with different conventional and super-resolution modalities. a Widefield vs dSTORM using Atto647N. b Confocal vs STED using Atto647N. c Widefield vs SIM using Alexa Fluor 488. d ExM prepared sample imaged with widefield vs e confocal using Alexa Fluor 488. The confocal ExM image furthermore shows the nucleus stained with Hoechst. Highlighted panels show × 3 zoomed areas. Scale bars in a–c are 1 μm, in d and e 10 μm and in the zoomed in areas 5 μm (after expansion)