Fig. 4.

Podocyte foot process (PFP) width as determined by the different super-resolution techniques. a An ExM image serves as an example in order to show the peak-to-peak distance of neighbouring fluorescence intensity maxima b in areas of hairpin-like distribution of nephrin that was considered equivalent to the width of PFPs (blue line in a). Scale bar 5 μm (after expansion). c PFP width as determined by SIM (n = 159), STED (n = 87), dSTORM (n = 36), and ExM^N/ExM^G (n = 210); expansion factor determined by nuclear and glomerular expansion, respectively. Bars represent mean ± SD