TABLE 2.

Engineering strategies to improve fatty acid-based bioproducts accumulation in Y. lipolytica.

Engineering strategies	Bioproducts	Strategy details	References
Constructing and engineering metabolic pathways	EPA	Constructing synthetic pathways	Xue et al., 2013
	Lipids	Improving acetyl-CoA supplement	Xu et al., 2016
	Lipids	Increasing NADPH availability	Qiao et al., 2017
	Trans-10, cis-12 CLA	Overexpressing the endogenous enzymes	Wang et al., 2019
	Fatty alcohols	Eliminating downstream degradation	Rutter and Rao, 2016
Optimizing fermentation conditions	γ-decalactone	Improving oxygen transfer	Moradi et al., 2013
	GLA	A temperature-shift strategy of cultivation	Sun et al., 2017
	CLA	Changing the medium components	Wang et al., 2019
	EPA	Two-stage continuous fermentation	Xie et al., 2017
Engineering compartmentalization system	FAEE	Endoplasmic reticulum or peroxisome localization	Xu et al., 2016
	Alkane	Endoplasmic reticulum or peroxisome localization
	Fatty alcohol	Peroxisome localization
	γ-decalactone	Cell immobilization	Zhang et al., 2020