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. 2021 Jan 5;12:573714. doi: 10.3389/fnsyn.2020.573714

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Copyright © 2021 Kushnireva, Korkotian and Segal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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New protrusions are associated with STIM1. (A1) Puncta are shown at two time-points; t = 0 and (A2) t = 15 min. Top images are EGFP (green, for morphology) and STIM1-mCherry (red) merged. Bottom panels show the same fields with EGFP dimmed. Scale bar = 2 μm. (B) The overall ratio of STIM1-containing protrusions in 10, 15, and 20 DIV is shown in absolute values. The total number of protrusions per standard field (filopodia and spine-like structures) are shown for all three ages (n = 40 fields for each age). (C) The normalized proportion of STIM1-positive protrusions as a percent of the total number of protrusions per field. (D) The appearance of new protrusions, associated with STIM1 puncta for DIV 10, 15, and 20 in the standard field per 30 min is summarized. **Very significant, 0.01 > p > 0.001; ***highly significant, p < 0.001.