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. 2021 Jan 5;11:565557. doi: 10.3389/fendo.2020.565557

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Copyright © 2021 Hoeflich, Fitzner, Walz, Hecker, Tuchscherer, Brenmoehl and Zettl

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chart of a treatment cycle and sample collection. Multiple sclerosis (MS) patients suffering from spasticity were treated with intrathecal triamcinolone acetonide (TCA) every 3 months. Each treatment cycle consisted of four TCA applications (usually every other day). The patients were lumbar-punctured, and to avoid CSF pressure differentials, the CSF was drained and replaced with the same amount of TCA solution. After the procedure, peripheral blood was drawn for routine laboratory analyses. Remaining sample material was stored in a biobank at −80°C.