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. 2021 Jan 18;11:1732. doi: 10.1038/s41598-021-81239-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transgenic ascl1b-2A-Cre, olig2-2A-Cre and neurod1-2A-Cre lines express functional Cre that promotes recombination at loxP sites in the expected neural progenitor populations. (a) F0 adults harboring precise integration alleles were mated to the recombination reporter line ubi:Switch to generate double transgenic 2A-Cre driver; ubi:Switch embryos. (b–d) Confocal imaging of 3 dpf double transgenic ascl1b-2A-Cre; ubi:switch (b), olig2b-2A-Cre; ubi:switch (c), and neurod1-2A-Cre; ubi:switch (d) larvae shows a switch from GFP to mCherry expression in neural cells derived from ascl1b, olig2, and neurod1 progenitors. (e) A switch from GFP to mCherry expression isn’t detected in single transgenic ubi:Switch larvae. fb forebrain, γ-cry gamma crystallin promoter, EGFP green fluorescent protein, hb hindbrain, mb midbrain, nt neural tube, op olfactory placode, r retina. Scale bar 200 μm.