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. 2021 Jan 18;11:1732. doi: 10.1038/s41598-021-81239-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ascl1b-2A-Cre, olig2-2A-Cre and neurod1-2A-Cre Cre recombinase activity in the 3 dpf larval midbrain and hindbrain. F3 or F2 adults were mated to the recombination reporter line ubi:Switch to generate double transgenic embryos. Confocal imaging of ascl1b-2A-Cre; ubi:Switch (a–a′′,b–b′′), control sibling ubi:Switch (c,d), olig2-2A-Cre; ubi:Switch (e–e′′,f–f′′) and neurod1-2A-Cre; ubi:Switch (g–g′′,h–h′′) larval midbrain tectum (t), cerebellum (c) and hindbrain (h). Arrows point to the ventricular zone lining the midbrain and hindbrain ventricles (v). Expression of ascl1b-2A-Cre earlier in brain development leads to nearly all descendant neurons expressing mCherry (a,b). No switching occurs in the absence of Cre (c–c′′,d–d′′). olig2-2A-Cre leads to mCherry expression in a subset of cells in the cerebellum (arrowheads), and neural progenitors lining the hindbrain ventricular zone and their descendants (e,f). In neurod1-2A-Cre mCherry expression is absent from the ventricular zone (v arrows) but present throughout neurons in the tectum, cerebellum (arrowheads) and hindbrain (g,h). Scale bars 50 μm.