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. 2021 Jan 18;11:1732. doi: 10.1038/s41598-021-81239-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Tamoxifen regulated CreERT2 recombinase activity in F0 somatic targeted embryos. (a) nacre; ubi:Switch embryos injected with Cas9mRNA, sgRNAs and the neurod1-2A-CreERT2 targeting vector. At 6 hpf embryos were mock treated or treated with 5um 4-hydroxytamoxifen and imaged at 3 dpf. (b–b′′) Injected embryos without treatment or (c–c′′) mock treated show a low level of expression in the brain. (d–d′′) 4-hydroxytamoxifen treatment resulted in switching from EGFP to mCherry expression in cells in the midbrain, hindbrain and neural tube. Arrows point to off-target expression. (e–e′′) Dorsal imaging of the brain in the larvae shown in d–d′′ revealed mCherry expression was detected in cells throughout the midbrain and hindbrain parenchymal tissue. 4-OHT 4-hydroxytamoxifen, c cerebellum, EGFP enhanced green fluorescent protein, hb hindbrain, mb midbrain, nt neural tube, t optic tectum, v ventricle. Scale bars (b′′,c′′,d′′) 200 μm; (e′′) 50 μm.