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. 2021 Jan 5;8:572356. doi: 10.3389/fbioe.2020.572356

FIGURE 2.

FIGURE 2

PTHrP does not affect Safranin-O staining in cocultured pellets with interaction index > 1 (i.e., Responders). After 3 weeks of culture in chondrogenic media, pellets were fixed, embedded, and sectioned for histology. (A) The GAG per DNA contents of the monoculture and cocultured pellets associated with interaction index > 1. (B) The interaction index of cocultured pellets. (C–H) Safranin O staining of pellets formed from 250,000 cells and (I–N) Safranin O staining of pellets formed from 500,000 cells. The main and inset images are at 200x and 100x optical magnification, respectively. Scale bars are 100 μm. Statistical significance was determined using one-way ANOVA with Tukey’s HSD post hoc test (three donors in total; two donors in duplicates at 250,000 cells per pellet and one donor in duplicates at 500,000 cells per pellet; n = 6; ***p < 0.001).